| 出品公司: | ATCC |
|---|---|
| 菌种名称: | ATCC CRL-1721, ATCC CRL-1721 |
| 菌株类型: | PC-12 |
| 存储人: | B Patterson |
| 培养物性质: | 漂浮的簇；少数散在的轻附着的细胞。 |
| 产品目录号: | CRL-1721 |
| 低温保存: |
冷冻培养基：添加10%（v/v）二甲基亚砜的完整生长培养基
储存温度：液氮汽相
|
| 培养基: |
该细胞系的基本培养基是ATCC配方的RPMI-1640培养基，目录号30-2001。为制备完整的生长培养基，向基础培养基中添加以下成分：
将灭活马血清加热至10%的最终浓度
最终浓度为5%的胎牛血清
|
| 生长条件: |
大气：空气，95%；二氧化碳（CO2），5%
温度：37°C
|
| 生物安全等级: | 1 |
| 储存条件: | 液氮汽相 |
| 应用: | 该细胞系是合适的转染宿主。 |
| 菌株特点: |
ATCC CRL-1721是PC-12 |
| 参考文献: |
Levi A, et al. Molecular cloning of a gene sequence regulated by nerve growth factor. Science 229: 393-395, 1985. PubMed: 3839317
Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897
Biocca S, et al. A macromolecular structure favouring microtubule assembly in NGF- differentiated pheochromocytoma cells (PC12). EMBO J. 2: 643-648, 1983. PubMed: 6641712
Weber E, et al. Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells. J. Biol. Chem. 271: 6963-6971, 1996. PubMed: 8636125
U.S. Pharmacopeia USP Monographs: Small Intestinal Submucosa Wound Matrix. Rockville, MD. USP32-NF27, 2005
Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897
|
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